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Yang YU, Jiafu TANG
《工程管理前沿(英文)》 2019年 第6卷 第2期 页码 183-192 doi: 10.1007/s42524-019-0028-1
关键词: seru production production mode flexibility literature review
Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis
《化学科学与工程前沿(英文)》 2023年 第17卷 第4期 页码 425-436 doi: 10.1007/s11705-022-2229-0
关键词: acetoin pyruvate α-acetolactate synthetase α-acetolactate decarboxylase Halomonas bluephagenesis whole-cell biocatalysis
Rhamnolipid synthesis and production with diverse resources
Qingxin Li
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 27-36 doi: 10.1007/s11705-016-1607-x
关键词: biosurfactant rhamnolipid Pseudomonas waste surface tension
Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric
《化学科学与工程前沿(英文)》 2022年 第16卷 第12期 页码 1751-1760 doi: 10.1007/s11705-022-2211-x
关键词: ARTP mutagenesis Bacillus licheniformis poly-γ-glutamic acid metabolomics
Shen Huang,Xudong Feng,Chun Li
《化学科学与工程前沿(英文)》 2015年 第9卷 第4期 页码 501-510 doi: 10.1007/s11705-015-1544-0
关键词: β-glucuronidase glycyrrhetic acid monoglucuronide cell disruption purification chromatography
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
《农业科学与工程前沿(英文)》 2020年 第7卷 第4期 页码 513-522 doi: 10.15302/J-FASE-2019272
is a shrub that has been used in traditional herbal medicine. It has great pharmaceutical potential for treating diseases such as cancer and diabetes. As a prerequisite for propagation of this species on a large scale, hairy roots in were induced using and various factors affecting hairy root induction and growth evaluated. Seven factors were tested: (1) type of explant, (2) type of culture medium, (3) duration of pre-culture, (4) inoculum cell density, (5) duration of infection, (6) acetosyringone concentration in the culture medium, and (7) duration of incubation. The optimal protocol for hairy root induction and growth was: young shoots, pre-cultured in Y1 for 2 d, inoculated with broth with an OD of 0.6 for 20 min, and incubated for 3 d. Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction. Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of . Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.
关键词: hairy root induction medicinal plant MS growth medium Phyllanthus hainanensis Rhizobium rhizogenes
Production of rhamnolipids-producing enzymes of
Kata Kiss,Wei Ting Ng,Qingxin Li
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 133-138 doi: 10.1007/s11705-017-1637-z
关键词: rhamnolipids Pseudomonas RhlA RhlB RhlI protein folding
High production of butyric acid by
Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu
《化学科学与工程前沿(英文)》 2015年 第9卷 第3期 页码 369-375 doi: 10.1007/s11705-015-1525-3
关键词: Clostridium tyrobutyricum butyric acid production fermentation mutant pH flux balance analysis
Marukawa, I. Hirasawa
《化学科学与工程前沿(英文)》 2013年 第7卷 第1期 页码 55-59 doi: 10.1007/s11705-013-1312-y
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
《化学科学与工程前沿(英文)》 2018年 第12卷 第4期 页码 806-814 doi: 10.1007/s11705-018-1739-2
The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.
关键词: synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae
《化学科学与工程前沿(英文)》 2023年 第17卷 第8期 页码 1109-1121 doi: 10.1007/s11705-022-2296-2
关键词: life-cycle assessment techno-economic analysis wood vinegar activated carbon Eucommia
《环境科学与工程前沿(英文)》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1637-9
● Converting xylose to caproate under a low temperature of 20 °C by MCF was verified.
关键词: Xylose fermentation Caproate Low temperature Bifidobacterium FAB pathway RBO pathway
《化学科学与工程前沿(英文)》 2021年 第15卷 第6期 页码 1561-1571 doi: 10.1007/s11705-021-2089-z
关键词: three-dimensionally ordered macroporous SrTiO3 ZnxCd1–xS visible light hydrogen production promotion mechanism
利用<i>cyp17a1i>缺失的伪雄鲤成功构建全雌鲤群体 Article
翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战
《工程(英文)》 2022年 第8卷 第1期 页码 181-189 doi: 10.1016/j.eng.2021.03.026
部分养殖鱼类存在两性生长异形的表型,因此,构建相应单性别养殖群体对水产养殖产量的提升具有产业应用价值。迄今为止,采用激素活性物质诱导产生性反转亲本是水产养殖业构建单性别群体最普遍使用的方法。本研究针对雌鲤生长快于雄鲤的两性生长异形的特性,以cyp17a1 为靶点进行了CRISPR/Cas9介导的基因编辑操作,利用cyp17a1 敲除后形成的伪雄鲤,成功构建了全雌鲤群体。首先,我们发现无论遗传性别型为XX还是XY,其cyp17a1 缺失鲤个体性腺均发育为精巢,并能正常产精。其次,利用确定的雄性特异DNA分子标记,筛选到cyp17a1 -/- XX伪雄个体,其具有正常的精巢发育和精子发生。将其与野生型雌鲤杂交,在8 月龄时检测后代性别,发现100%为雌性。将全雌群体和对照组雌雄混合群体各500 尾进行同塘生长对比养殖,至8 月龄或12 月龄时随机抽样检测,发现cyp17a1杂合突变雌鲤体重比同塘性别混养对照组分别高6.60%或32.66%。因此,本研究首次成功地利用基因工程技术对单基因位点进行操作,创制了全雌群体,实现了对雌鲤生长快的两性生长异形特性的应用,提升了养殖产量。
Multi-stage ammonia production for sorption selective catalytic reduction of NO
Chen ZHANG, Guoliang AN, Liwei WANG, Shaofei WU
《能源前沿(英文)》 2022年 第16卷 第5期 页码 840-851 doi: 10.1007/s11708-021-0797-1
关键词: selective catalytic reduction (SCR) nitrogen oxides (NOx) ammonia composite sorbent chemisorption
标题 作者 时间 类型 操作
Genomic and metabolomic analysis of with enhanced poly--glutamic acid production through atmospheric
期刊论文
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
期刊论文
High production of butyric acid by
Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu
期刊论文
Filtration ability of hollow fiber membrane for production of magnesium ammonium phosphate crystals by
Marukawa, I. Hirasawa
期刊论文
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
期刊论文
Life-cycle assessment and techno-economic analysis of the production of wood vinegar from stem: a case
期刊论文
Low-temperature caproate production, microbial diversity, and metabolic pathway in xylose anaerobic fermentation
期刊论文
Superior performance in visible-light-driven hydrogen evolution reaction of three-dimensionally ordered macroporous SrTiO decorated with ZnCdS
期刊论文